Competitive Fitness Assays Indicate that the E138A Substitution in HIV-1 Reverse Transcriptase DecreasesIn VitroSusceptibility to Emtricitabine

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HIV-1 clinical isolates with the E138A substitution in reverse transcriptase show full susceptibility to emtricitabine and other nucleoside reverse transcriptase inhibitors.

Arecent report by Sluis-Cremer and colleagues found that HIV-1 site-directed mutants containing the E138A substitution in reverse transcriptase (RT) exhibited decreased susceptibility to the nucleoside reverse transcriptase inhibitors (NRTIs) emtricitabine (FTC) and lamivudine (3TC) in vitro by 4.7-fold and 6.0-fold, respectively, compared to wild-type virus (1). Other amino acid substitutions ...

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Role of the K101E substitution in HIV-1 reverse transcriptase in resistance to rilpivirine and other nonnucleoside reverse transcriptase inhibitors.

Resistance to the recently approved nonnucleoside reverse transcriptase inhibitor (NNRTI) rilpivirine (RPV) commonly involves substitutions at positions E138K and K101E in HIV-1 reverse transcriptase (RT), together with an M184I substitution that is associated with resistance to coutilized emtricitabine (FTC). Previous biochemical and virological studies have shown that compensatory interaction...

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Aptamers that recognize drug-resistant HIV-1 reverse transcriptase

Drug-resistant variants of HIV-1 reverse transcriptase (RT) are also known to be resistant to anti-RT RNA aptamers. In order to be able to develop diagnostics and therapies that can focus on otherwise drug-resistant viruses, we have isolated two aptamers against a well-known, drug-resistant HIV-1 RT, Mutant 3 (M3) from the multidrug-resistant HIV-1 RT panel. One aptamer, M302, bound M3 but show...

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Human immunodeficiency virus (HIV-1) is, like most pathogens, under selective pressure to escape the immune system of its host. In particular, HIV-1 can avoid recognition by cytotoxic T lymphocytes (CTLs) by altering the binding affinity of viral peptides to human leukocyte antigen (HLA) molecules, the role of which is to present those peptides to the immune system. It is generally assumed that...

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ژورنال

عنوان ژورنال: Antimicrobial Agents and Chemotherapy

سال: 2014

ISSN: 0066-4804,1098-6596

DOI: 10.1128/aac.02114-13